Determination of tanshinone IIA in Salvia miltiorrhiza Bunge extract and its effect on ischemia-reperfusion injury
نویسندگان
چکیده
To establish an HPLC method for determination of tanshinone IIA in Salvia miltiorrhiza Bunge extract, and observe the protective effect of Salvia miltiorrhiza extract on myocardial ischemia-reperfusion injury in rats. Column used is a Diamonsil C18, mobile phase is methanol-water (75:25), and detection wavelength is 270 nm. Ischemia-reperfusion injury model is replicated, and the size of myocardial infarction area is observed, as well as myocardial pathological changes under light microscope (LM) and transmission electron microscope (TEM). Tanshinone IIA shows a good linearity (r=0.9999) within 0.074~0.37 μg, and its recovery is 99.17% (RSD 1.76%, n=6). In Salvia miltiorrhiza extract group, myocardial infarction area shrinks markedly, and the degree of myocardial cell degeneration and necrosis, and ultrastructural morphological changes in myocardial cells are significantly reduced under LM and TEM. The method established is simple and accurate. Salvia miltiorrhiza extract has a protective effect on myocardial ischemia-reperfusion injury in rats.
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